Composite

Part:BBa_K4092101:Design

Designed by: Damian Alonso Espinoza Ruiz & Shani Betsymar Leyva Camacho   Group: iGEM21_Tec-Monterrey   (2021-10-18)


Toehold Agave 1 expression cassette with mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 681
    Illegal AgeI site found at 793
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This cassette expression is regulated by a T7 promoter and expresses a red fluorescent protein when the toehold interacts with its corresponding trigger sequence.



Source

Toehold RNA complementary to SST1 (sucrose 1-fructosyltransferase) from Agave tequilana, T7 promoter from bacteriophage T7 and mRFP from Discosoma striata (coral).

References